Target enrichment (Other Keyword)

DOCUMENT Citation Only Jacob Enk.

Bait-target hybridization (a.k.a., "target capture") is rapidly replacing PCR as the enrichment method of choice for ancient DNA sequencing projects. Though very successful in recent years, ancient DNA target capture outcomes vary substantially and could be better understood. Here we performed a series of experiments to measure how three commonly-varied parameters - temperature, time, and bait tiling density - impact enrichment of short, rare targets embedded in complex DNA backgrounds. We found...