ZooMS, collagen, archaeology, leather, tannins (Other Keyword)
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A Method to Extract Collagen from Archaeological Leather for Species Identification with ZooMS (2017)
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Zooarchaeology by Mass Spectrometry (ZooMS) is a rapid peptide fingerprinting technique capable of identifying species provenance in several archaeological materials of biological origin, and most commonly used on bone. Leather has proven resistant to analysis not only by ZooMS, but also to DNA extraction due to the tannins that are present in the material. We have used alkali (NaOH) to increase the solubility of the tannins and thereby extract them before enzyme digestion. This has allowed us...