Next-generation sequencing (Other Keyword)
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This is an abstract from the "SAA 2023: Individual Abstracts" session, at the 88th annual meeting of the Society for American Archaeology. Ancient DNA (aDNA) analysis can be a useful tool for sex determination, general mitochondrial lineage (haplogroup), and disease diagnosis in human remains. However, non-endogenous DNA contamination of archaeological material is a recurrent problematic, since excavation, handling, and storage usually don’t fit with the precautions recommended for aDNA...
Target Capture for Ancient DNA: Temperature, Time, and Tiling Density (2016)
Bait-target hybridization (a.k.a., "target capture") is rapidly replacing PCR as the enrichment method of choice for ancient DNA sequencing projects. Though very successful in recent years, ancient DNA target capture outcomes vary substantially and could be better understood. Here we performed a series of experiments to measure how three commonly-varied parameters - temperature, time, and bait tiling density - impact enrichment of short, rare targets embedded in complex DNA backgrounds. We found...