STUDY OF LEISHMANIASIS IN ARCHAEOLOGICAL MATERIAL OF HUMAN ORIGIN FROM SOUTH AMERICAN ARCHAEOLOGICAL SITES
The identification of Leishmania parasites in archaeological material is performed by molecular and immunological diagnosis.The present study aimed to detect Leishmania sp. in samples from archaeological sites in South America.After the lack of inhibition observed in the samples we proceeded with PCR Leishmania spp.using a molecular target to Kinetoplast minicircule kDNA in samples from different individuals with datings from different periods from archaeological sites in South America(Brazil and Chile).A total of 46 samples of tissues and bones from different were collected,the DNA was extracted in 33 and the PCR were performed in 19.The results were negative for 7 samples and positive for 2 samples of a naturally mummified human body found in Minas Gerais state,Brazil,dating from 1700 to 1800 AD showing similarity of 100% in two samples for Leishmania tarentolae,a species belonging to the subgenus Sauroleishmania found in lizards and first isolated from the Moorish gecko Tarentolae mauritanica.This specie isn’t pathogenic to humans and hasn’t yet been diagnosed therein.To confirm these results,a new molecular analysis was performed on bone marrow sample with 100% similarity to L. tarentolae,which confirmed the infection by this parasite.This result isn’t a false parasitism, since the analysis was performed directly on tissue samples and bone marrow.
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STUDY OF LEISHMANIASIS IN ARCHAEOLOGICAL MATERIAL OF HUMAN ORIGIN FROM SOUTH AMERICAN ARCHAEOLOGICAL SITES. Shênia Novo, Daniela Leles, Raffaella Bianucci, Adauto Araújo. Presented at The 80th Annual Meeting of the Society for American Archaeology, San Francisco, California. 2015 ( tDAR id: 397579)
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min long: -93.691; min lat: -56.945 ; max long: -31.113; max lat: 18.48 ;