STUDY OF LEISHMANIASIS IN ARCHAEOLOGICAL MATERIAL OF HUMAN ORIGIN FROM SOUTH AMERICAN ARCHAEOLOGICAL SITES

Author(s): Shênia Novo; Raffaella Bianucci; Daniela Leles; Adauto Araújo

Year: 2015

Summary

The identification of Leishmania parasites in archaeological material is performed by molecular and immunological diagnosis.The present study aimed to detect Leishmania sp. in samples from archaeological sites in South America.After the lack of inhibition observed in the samples we proceeded with PCR Leishmania spp.using a molecular target to Kinetoplast minicircule kDNA in samples from different individuals with datings from different periods from archaeological sites in South America(Brazil and Chile).A total of 46 samples of tissues and bones from different were collected,the DNA was extracted in 33 and the PCR were performed in 19.The results were negative for 7 samples and positive for 2 samples of a naturally mummified human body found in Minas Gerais state,Brazil,dating from 1700 to 1800 AD showing similarity of 100% in two samples for Leishmania tarentolae,a species belonging to the subgenus Sauroleishmania found in lizards and first isolated from the Moorish gecko Tarentolae mauritanica.This specie isn’t pathogenic to humans and hasn’t yet been diagnosed therein.To confirm these results,a new molecular analysis was performed on bone marrow sample with 100% similarity to L. tarentolae,which confirmed the infection by this parasite.This result isn’t a false parasitism, since the analysis was performed directly on tissue samples and bone marrow.

SAA 2015 abstracts made available in tDAR courtesy of the Society for American Archaeology and Center for Digital Antiquity Collaborative Program to improve digital data in archaeology. If you are the author of this presentation you may upload your paper, poster, presentation, or associated data (up to 3 files/30MB) for free. Please visit http://www.tdar.org/SAA2015 for instructions and more information.

Cite this Record

STUDY OF LEISHMANIASIS IN ARCHAEOLOGICAL MATERIAL OF HUMAN ORIGIN FROM SOUTH AMERICAN ARCHAEOLOGICAL SITES. Shênia Novo, Daniela Leles, Raffaella Bianucci, Adauto Araújo. Presented at The 80th Annual Meeting of the Society for American Archaeology, San Francisco, California. 2015 ( tDAR id: 397579)

This Resource is Part of the Following Collections

Keywords

General
ancient DNA • Leishmania • Lizards

Geographic Keywords
South America

Spatial Coverage

min long: -93.691; min lat: -56.945 ; max long: -31.113; max lat: 18.48 ;