Validation of a Non-Destructive DNA Extraction Protocol for Ancient DNA Analyses
The destructive nature of traditional DNA extraction techniques presents one of the primary obstacles to accessing genetic information from museum and archaeological collections. Here we assess a recently published "non-destructive" DNA extraction protocol by Bolnick and colleagues in terms of the amount and quality of DNA extracted from a set of samples of even greater antiquity than those tested in the original analysis. DNA was successfully extracted from archaic period samples from the Eva site in Tennessee. Both quantity and quality of DNA extracted were high enough to obtain mitochondrial sequence data using traditional (Sanger) sequencing. Two samples were successfully amplified using a whole mitochondrial genome amplification kit and underwent next generation sequencing. We also show that the method is indeed "non-destructive." Dental metrics and weights taken before and after extraction indicate no significant change in the teeth due to the extraction process. Thus, the use of this protocol could not only expand the range of samples available for DNA analysis, but also the amount of genetic information obtained from the analysis.
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Validation of a Non-Destructive DNA Extraction Protocol for Ancient DNA Analyses. Frankie Pack, Kathryn Kulhavy, Graciela Cabana. Presented at The 80th Annual Meeting of the Society for American Archaeology, San Francisco, California. 2015 ( tDAR id: 398067)
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